KishanganjBihar.com is delighted over sharing the news of Seemanchal's pride Raghibul Hasan, a Senior Research Fellow (SRF) at Department of Biochemistry in All India Institute of Medical Sciences (AIIMS), New Delhi. A resident of Katihar district, Raghibul Hasan has done an exceptional research to determine the functional significance of TC21 in esophageal squamous cell carcinoma (ESCC). His research brought the conclusion that TC21 mediates its effects via the PI3K-Akt pathway, NF-κB and cyclin D1, and enhances chemoresistance in esophageal cancer cells. This is really a big achievement for Raghibul Hasan as he belong to one of the most educationally backward regions of the country. In fact, Raghibul Hasan's effort will encourage the youth of Seemanchal to join the research field as nowadays very few chose it as career option.
Abstract of Raghibul Hasan's paper online edition.
siRNA-mediated downregulation of TC21 sensitizes esophageal cancer cells to cisplatin.
Md. Raghibul Hasan, Shyam Singh Chauhan, Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India.
AIM: To determine the functional significance of TC21 in esophageal squamous cell carcinoma (ESCC).
METHODS: TC21 siRNA transfection was carried out using Hyperfectamine to knock down TC21, and transcripts were analyzed by reverse transcription-polymerase chain reaction and protein by Western blotting. We demonstrated the effect of TC21 downregulation of cell signaling in esophageal cancer cells by assessing the phosphorylation status of its downstream targets, phosphoinositide 3-kinase (PI3K), phosphatase and tensin homolog (PTEN), protein kinase B (pAkt), nuclear factor-κB (NF-κB) and cyclinD1 using specific antibodies. Cell survival analysis after cisplatin treatment was carried out by cell viability assay and cell cycle analysis using flow cytometry.
RESULTS: TC21 knockdown in human ESCC cell line TE13 cells, showed only a marginal increase (14.2%) in cell death compared with control cells. The expressions of the signaling proteins PI3K and pAkt, transcription factor NF-κB, and cell cycle protein cyclin D1 were markedly decreased in response to TC21 downregulation, whereas the level of pPTEN, an antagonist of PI3K, was increased. In addition, we evaluated the potential of TC21 as a putative target for sensitizing ESCC cells to the chemotherapeutic agent cisplatin. Increased cell death (38.4%) was observed in cells treated with cisplatin after TC21 knockdown compared with cells which were treated with cisplatin alone (20% cell death).
CONCLUSION: Results suggest that TC21 mediates its effects via the PI3K-Akt pathway, NF-κB and cyclin D1, and enhances chemoresistance in esophageal cancer cells.
